metalloproteinase timp 1  (R&D Systems)

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 4 TIMP1 secreted by macrophage is involved in the regulation of adipogenesis. (A) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (B) Concentration of TIMP1 were quantified. (C) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (D) Oil red O stain of 3T3-L1 in different concentration of TIMP1. (E) mRNA expression of TIMP1 in adipocyte and macrophage. (F) Representative IHC staining of F4/80 in SAT and VAT from WT and Rarres2/ mice. All the mice were male and fed with HFD for 12 weeks if not indicated otherwise, n Z 6e8/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Concentration Assay, Staining, Expressing, Immunohistochemistry

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 5 Knock down of chemerin specific in SAT promote adipogenesis and improve GTT and ITT. Male mice were fed with HFD and infected with shLacZ or shRarres2 twice weekly via subcutaneous injection adjacent to inguinal SAT for 8 weeks. (A) Gene expression levels of chemerin in SAT. (B) Body weight of mice. (C) Comparison of SAT. (D) Ratio of fat weight to body weight. (E) Total DNA of SAT. (F and H) Adipocyte cell size of SAT. (F and G) IHC of TIMP1 in SAT. (I) mRNA expression levels of TIMP1 in SAT. (J) Western blot analysis of PPARg and C/EBPa. (K) GTT and ITT analysis. (L-M) Representative flow cytometric analysis of adipocyte- progenitors (L) and macrophage (M) in SAT of indicated mice. n Z 6/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Knockdown, Infection, Injection, Gene Expression, Comparison, Expressing, Western Blot

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 4 TIMP1 secreted by macrophage is involved in the regulation of adipogenesis. (A) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (B) Concentration of TIMP1 were quantified. (C) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (D) Oil red O stain of 3T3-L1 in different concentration of TIMP1. (E) mRNA expression of TIMP1 in adipocyte and macrophage. (F) Representative IHC staining of F4/80 in SAT and VAT from WT and Rarres2/ mice. All the mice were male and fed with HFD for 12 weeks if not indicated otherwise, n Z 6e8/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Concentration Assay, Staining, Expressing, Immunohistochemistry

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 5 Knock down of chemerin specific in SAT promote adipogenesis and improve GTT and ITT. Male mice were fed with HFD and infected with shLacZ or shRarres2 twice weekly via subcutaneous injection adjacent to inguinal SAT for 8 weeks. (A) Gene expression levels of chemerin in SAT. (B) Body weight of mice. (C) Comparison of SAT. (D) Ratio of fat weight to body weight. (E) Total DNA of SAT. (F and H) Adipocyte cell size of SAT. (F and G) IHC of TIMP1 in SAT. (I) mRNA expression levels of TIMP1 in SAT. (J) Western blot analysis of PPARg and C/EBPa. (K) GTT and ITT analysis. (L-M) Representative flow cytometric analysis of adipocyte- progenitors (L) and macrophage (M) in SAT of indicated mice. n Z 6/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Knockdown, Infection, Injection, Gene Expression, Comparison, Expressing, Western Blot